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Tiwari, R. K. s.
- A Study on the Performance of In-vitro and In-vivo Germination of Propagatory Materials of Certain Medicinal Plants
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Indian Forester, Vol 133, No 7 (2007), Pagination: 979-985Abstract
In-vitro and in-vivo experiments were undertaken conducted to determine the germination of medicinal plant species through seeds and sprouting of cuttings. Seeds of 42 medicinal plant species prevalent in Chhattisgarh forests were selected for germination test in the laboratory as well as in the nursery. For vegetative propagation through cuttings, 44 species were selected. Out of 42 species tested, seed germination was above 90 per cent in species like Aparajita (Clitoria ternatea), Hinglaj (Cassia alata), Jungli sem (Canavalia gladiata), Kasoundhi (Cassia occidentalis), Kenwach (Mucuna pruriens), Ram tulsi (Ocimum basilicum) and Vansuran (Amorphophallus sylvalicus). Whereas, seeds of species i.e. Bharangi (Clerodendrum serratum), Vaividang (Embelia tsjeriam) and Malkangni (Celastrus peniculatus) did not germinate either in the laboratory or in the nursery. Per cent sprouting and survival of the plants from stem cuttings were highest in Hanumansand (Lippia javanica) followed by Gandh prasarni (Paederia foetida), Chitawar (Baliospermum montanum) and Gudmar (Gymnena sylvestre).
- Isolation of Cold Tolerant Antifungal Strains of trichoderma sp. from Northern Hilly Zones of Chhattisgarh
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1 Department of Biotechnology, Barkatullah University, Bhopal M.P., IN
2 Department of Plant Pathology, T.C.B. College of Agriculture and Research Station, (I.G.A.U.), Ballarpur C.G., IN
1 Department of Biotechnology, Barkatullah University, Bhopal M.P., IN
2 Department of Plant Pathology, T.C.B. College of Agriculture and Research Station, (I.G.A.U.), Ballarpur C.G., IN
Source
International Journal of Plant Protection, Vol 6, No 2 (2013), Pagination: 236-240Abstract
Three species of Trichoderma viz., Trichoderma viride, Trichoderma harzianumand Trichoderma konengii have been isolated from the soil samples collected from forest sites in higher altitudes of Northern Hilly Zones of Chhattisgarh in Mainpat, Dist-Surguja, Chhattisgarh. The species could grow between 4 to 420 C temperatures and 3 to 13 pH on agar plates, the optimum requirement being 260 C and 5.5 Ph, respectively. Further incubation of the agar plates showing normal growth of Trichoderma species at 40C, induced heavy sporulation in three weeks of time. Induction of sporulation on exposure to low temperature appeared to be a strategy for survival of these species in extreme cold environment experiencing sub zero temperatures. Antifungal activities were demonstrated between Trichoderma species and phytopathogenic fungi in dual cultures. The antifungal metabolites produced by Trichoderma species, diffusible as well as volatile, caused abnormalities in fungal structures of pathogenic fungi. Plant growth promotion abilities of Trichoderma species was also demonstrated through a plant based bioassay in greenhouse. The study is important for documentation of microbial diversity of Northern Hilly Zones of Chhattisgarh in Mainpat, Dist-Surguja, Chhattisgarh and determination of the associated biotechnological applicationsKeywords
Trichoderma Species, Cold Tolerance, Antifungal- Efficacy of Bio-Agents against Damping Off in Solanaceous Crops under Nursery Conditions
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1 Krishi Vigyan Kendra (I.G.K.V.), Sarkanda Farm, Bilaspur (C.G.), IN
2 T.C.B. College of Agriculture and Research Station (I.G.K.V.), Sarkanda, Bilaspur (C.G.), IN
3 S.K. College of Agriculture, Kabirdham, Kawardha (C.G.), IN
1 Krishi Vigyan Kendra (I.G.K.V.), Sarkanda Farm, Bilaspur (C.G.), IN
2 T.C.B. College of Agriculture and Research Station (I.G.K.V.), Sarkanda, Bilaspur (C.G.), IN
3 S.K. College of Agriculture, Kabirdham, Kawardha (C.G.), IN
Source
International Journal of Plant Protection, Vol 11, No 1 (2018), Pagination: 1-9Abstract
The study was conducted to assess efficacy of Trichoderma harzianum and Pseudomonas fluorescens against damping off in tomato and brinjal seedlings during Kharif season. The investigation was performed as on farm trials on farmers’ field over three years viz., 2013-14, 2014-15 and 2015-16. T. harzianum and P. fluorescens were applied in combination of soil+seed treatment. Talc based formulation of both bio control agents were used @ 10 g kg-1 in seed treatment and 100 g m-2 in soil treatment. Results indicated that the combination of soil+seed treatment with T. harzianum was gives maximum seedling stands (84.15%) with minimum mortality (15.85%) in brinjal followed by soil+seed treatment with mixture of T. harzianum and P. fluorescens (78.30%) and soil+seed treatment with P. fluorescens alone (69.68%) whereas, in control plot treated with Carbendazim 50 WP (soil+seed) recorded less seedling stand (41.50%). In case of tomato, application of T. harzianum as soil+seed gave the maximum seedling stand (80.32%) followed by soil+seed treatment with combination of T. harzianum and P. fluorescens (73.45%) and P. fluorescens alone (58.64%) while seed+soil treatment with Carbendazim 50 WP given minimum seedling stand (42.01%). Consortium of T. harzianum and P. fluorescens significantly increased the growth and yield attributes of brinjal and tomato crops i.e. plant height, number of branches plant-1, number of fruit plant-1 and fruit yield ha-1 followed by along Trichoderma and Pseudomonas. The study showed that T. harzianum and P. fluorescens are the potential bio control agents as compare to Carbendazim 50 WP and can be used effectively for the management of damping off in seedlings in nursery.Keywords
Tomato, Brinjal, Bio-Agents, Damping Off, Pseudomonas, Trichoderma.References
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